Friday 13 May 2011

Enzymes of DNA Replication

Helicase: Unwounds a portion of the DNA Double Helix

RNA Primase: Attaches RNA primers to the replicating strands.

DNA Polymerase delta (ä): Binds to the 5' - 3' strand in order to bring nucleotides and create the daughter leading strand.

DNA Polymerase epsilon (å): Binds to the 3' - 5' strand in order to create discontinuous segments starting from different RNA primers.

Exonuclease (DNA Polymerase I): Finds and removes the RNA Primers

DNA Ligase: Adds phosphate in the remaining gaps of the phosphate - sugar backbone

Nucleases: Remove wrong nucleotides from the daughter strand.

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